WebAug 16, 2024 · Genome editing is an unprecedented technological breakthrough but low plant regeneration frequencies and genotype dependence hinder its implementation for crop improvement. Here, we found that transient expression of a complex of the growth regulators TaGRF4 and TaGIF1 (TaGRF4-TaGIF1) increased regeneration and genome … WebGRF4-GIF1 transgenic plants were fertile and without obvious developmental defects. Moreover, GRF4-GIF1 induced efficient wheat regeneration in the absence of exogenous …
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WebQin Feng, Ling Xiao, Yizhen He, Man Liu, Jiafa Wang, Shujuan Tian, Xian Zhang, and Li Yuan. "Highly efficient, genotype‐independent transformation and gene editing in watermelon (Citrullus lanatus) using a chimeric ClGRF4‐GIF1 gene" Journal of integrative plant biology 63, no. 12 (2024): 2038-2042. doi: 10.1111/jipb.13199 WebNov 11, 2024 · Highly efficient, genotype-independent transformation and gene editing in watermelon (Citrullus lanatus) using a chimeric ClGRF4-GIF1 gene. Feng Q, Xiao L, He Y, Liu M, Wang J, Tian S, Zhang X, Yuan L. J Integr … crossover black sandals
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WebDec 4, 2024 · Efficient genotype-independent genetic transformation in watermelon by overexpressing a chimeric ClGRF4-GIF1 gene (A) Schematic diagram of the vectors … WebOct 12, 2024 · The following day we added 300 μl of the Agrobacterium culture to 20 ml TY (pH 5.5) containing the appropriate antibiotics and 200 μM acetosyringone. We incubated the culture 28 °C for in a shaking incubator set at 250 rpm until the culture reached an OD 600 between 0.1 – 0.2 (approximately 2–4 h). WebDec 1, 2024 · ClGRF4-GIF1 can also be combined with clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) genome editing … crossover black friday