Ctg reagent
WebCell-Titer Glo (CTG) reagent (Promega) was dispensed at 4 µl/well using the BioRAPTR FRD Microfluidic Workstation. The plates were incubated in the dark at RT for 10 min prior to detection by the ViewLux plate reader (1 s exposure, 1× binning). To assess if stable expression of lux potentially WebMay 3, 2024 · Caspase-3/7 green detection reagent was used to monitor apoptosis over 12 hours (a,b), and the CTG assay was used to quantify live cells at 24 hours post-thawing (c).
Ctg reagent
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WebCytotoxicity Assays. Cytotoxicity is the degree to which a substance can cause damage to a cell. A substance or process that causes cell damage or death is referred to as cytotoxic, … WebHalf of the samples (n = 10) were treated with CTG reagent. (C) CTG measurement of untreated or tacrine-treated cells. Data are shown as mean ± SD values (n = 5). (D) Influence of CTG reagent on image quality. CTG reagent was removed from cells and the complete plate (CTG treated and untreated) was recorded by an Operetta HCS device …
WebalamarBlue HS and alamarBlue Cell Viability Reagents are ready-to-use, non-toxic, resazurin-based solutions that function as cell health indicators to quantitatively measure viability. alamarBlue uses the natural reducing power of living cells to convert resazurin to fluorescent resorufin. alamarBlue HS (High Sensitivity) Cell Viability Reagent is a … WebThe CellTiter-Glo® Luminescent Cell Viability Assay is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, an indicator of metabolically active cells. The CellTiter-Glo® Assay is designed for use … The CellTiter-Glo® Assay is designed for use with multiwell plate formats, making …
WebFor time course experiments using CTG, cells were incubated with the compounds from 2 to 72 h using a differ - ent plate per time point before adding 25 µL of CTG reagent. For time course experiments using the ImageTOX, cells were incubated with the compounds from 2 to 72 h, and the dye mixture was added 30 min before the first time point. WebMay 26, 2024 · Prepare the CTG reagent following the manufacturer’s instructions. 17. Remove the tissue culture plate from the incubator and leave it 10 min at room …
WebAbout us. Since first opening our doors in 1991, CTG has led the way in creating and implementing comprehensive technical solutions for media-centric markets. Whether installing state-of-the-art ...
WebThe CellTiter-Glo® 2.0 reagent is stable at room temperature and at 4°C, allowing fast and easy assay implementation, and eliminating the thawing and mixing required with other ATP assays. Enhanced stability … primary hlh diseaseWebMar 18, 2024 · Thaw and warm the CTG reagent. 18. Add CTG reagent at 1:3 ratio (50 μL per well of 150 μL culture media) to each well of the 96- well plate containing alginate beads by using a multichannel pipette. 19. Place the plates on a … primary holder accoubt tmobileWebAug 26, 2024 · Bring plates and CTG reagent to room temperature. 8. Dilute CTG reagent 1:1 with molecular grade water. Add 100 μL of diluted reagent to each well. Cover plate and shake plate for 5 min at room temperature. 9. Read luminescence following the protocol for the Synergy Neo2 Multi-Mode Microplate Reader (BioTek Instruments). primary hmo secondary ppoWebJan 30, 2015 · Importantly, to investigate the penetration of CTG reagent and its effect on schistosomula in the ATP assay, parasites treated with DMSO were incubated with the membrane-impermeant fluorogenic DNA-binding dye, CellTox green, previously added to the CTG reagent. Confocal laser fluorescent microscopy images showed robust … primary hlh treatmentWebNov 9, 2024 · Equilibrate CTG reagent to room temperature. Following degradation measurement at the last time point of the kinetic analysis, add 100 µL (96-well plate) or 40 µL (384-well plate) of the reagent per well of the plate, and mix on a plate shaker at 500-700 rpm (96-well plate) or a microplate vortex mixer (384-well plate) for 5 min. primary hlh genesWebMar 6, 2024 · A CTG file is an index file created by Canon digital cameras. It contains information used to organize images stored on a camera's memory card. For example, … primary hiv testsWebCytotoxicity is a general term that describes the detrimental effects of substances or environmental changes on cell health. Exposure of cells to a cytotoxic stimulus may compromise metabolic activity, inhibit cell growth or division or ultimately produce cell death. 'Necrotic' cell death is a catastrophic cell lysis. player animator minecraft