Fastgene gel/pcr extraction kit 300preps
Webhere - Nippon Genetics . here - Nippon Genetics . SHOW MORE WebPCR & Gel Extraction FastGene Gel/PCR Extraction Kit 100 preps FG-91202 $195 MEGAquick-spin plus Fragment DNA Purification Kit (from 87bp – 20kb) 200 preps …
Fastgene gel/pcr extraction kit 300preps
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WebSep 23, 2014 · I use PCR to amplify a 2.5-kbp gene and subsequently run the PCR products in 0.8% agarose gel. I get only a distinct band corresponding to my gene of interest. WebNucleic Acid Purification; PCR Instruments; PCR Setup & Liquid Handling; Pyrosequencing; Quality Control & Fragment Analysis; Service Solutions; Accessories; …
Web1.3 Cloning of the kelch13 PCR fragment ... purified using the FastGene Gel/PCR Extraction Kit (Nippon Genetics, Co., Ltd., Tokyo, Japan), and fused with the In-Fusion HD Cloning Kits (Takara
WebValidated for use with CGH labeling kit. PCR & Gel clean-up columns use silica membrane technology in a mini spin column format to deliver easy and efficient DNA purification from PCR products, agarose gels, and labeling reactions. Sample material: <400 µl PCR reaction mixture, <400mg agarose gel. Fragment size: 50bp - ~20kbp. Recovery: 70 - 95%. WebJan 2, 2024 · The specific products were purified using FastGene Gel/PCR Extraction kit (Nippon Gene) following the manufacturer’s protocol. For attP 54 (primer set: Dpro_5F1/Dpro_5R1) and attP 636 (primer sets: 3F1/3R1 and Dpro_5F1/5R1), two bands were detected, which were cut from the gel and purified using FastGene Gel/PCR …
WebThe purification and preparation of nucleic acids forms an important basis for many molecular biological analysis, in research institutes as well as medical laboratories. Our kits deliver high-quality nucleic acids (RNA, viral RNA/DNA, plasmid DNA, gel and PCR fragments) quickly and reliably. They can directly be used in subsequent applications.
Webagarose gel electrophoresis, followed by extraction using FastGene Gel/PCR Extraction kit (NIPPON Genetics, Japan). A quantity of 12 fmol purified EcoRV-digested pNGS2 was combined with 60 fmol of single-stranded oligonucleotides in a volume of 5 µ L. The oligonucleotides containing randomized N 12 (12-nucleotide sequences, 5'- shared parental leave form nhsWebgel/pcr エクストラクションキットのページです。製品概要、アプリケーションノート、オーダー情報、取扱説明書、技術資料、文献、msds等、 … shared parental leave employment lawWebJun 22, 2024 · PCR products from the previous reactions were purified from an agarose gel using a FastGene Gel/PCR Extraction Kit (Nippon Genetics, Tokyo, Japan) and used as templates for mass amplification using TB71/CI78. sgRNA was synthesized and purified using the same kit. The concentration of the DNA template and the sgRNA were … shared parental leave uk formsWebAlso, one can always run a few microliters of a PCR product or vector linearization on a gel to check for purity/complete digestion, and then do a PCR purification on the remaining volume. pool templates softwareWebMay 30, 2024 · PCR products were purified using a FastGene Gel/PCR Extraction Kit (Nippon Genetics). These samples were subjected to cycle sequencing using a BigDye™Terminator Cycle Sequencing ... A paired-end library was constructed with a TruSeq DNA PCR-Free Library Prep kit (Illumina, CA, USA) and was size-selected on … pooltester chlor und ph digitalWebCleanNGS SPRI Bead Purification Kit (277 clean-ups) 5 ml CNGS005 $124.69 PCR & Gel Extraction Kits FastGene Gel/PCR Extraction Kit 100 preps FG-91202 $194.51 Plasmid Purification Kit Fast DNA-spin Plasmid Purification Kit 200 preps 17013 $236.25 DNA-spin Plasmid Purification Kit 200 preps 17098 $162.75 DNA-maxi Endotoxin-Free … shared parental leave form for employerWebApr 27, 2024 · The FastGene ® RNA kits were developed for the rapid, efficient and clean purification of RNA from tissues and cells. The silica … shared parental leave process uk