Goldenticket mice
WebAug 21, 2024 · Pharmacological activation of the STING (stimulator of interferon genes)–controlled innate immune pathway is a promising therapeutic strategy for cancer. Here we report the identification of MSA-2, an orally available non-nucleotide human STING agonist. In syngeneic mouse tumor models, subcutaneous and oral MSA-2 regimens … WebSep 25, 2011 · Mouse Sting and the goldenticket (I199N) mutant allele of Sting were cloned into pcDNA3 with a carboxy terminal hemagglutinin (HA) tag as described previously 11. DNA encoding the carboxy terminal domain of mouse Sting (nucleotides 414 - 1137) was cloned into pET28a for recombinant protein expression in E. coli.
Goldenticket mice
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WebSTING-mutant goldenticket mice and found that the antitumor efficacy of STINGVAX required functional STING (Fig. 1E). In addition, STINGVAX efficacy was diminished in … WebFeb 2, 2024 · Shown are results for 7 to 13 mice per genotype pooled from 3 independent experiments. (B) Kaplan-Meier curve showing mortality rates of 7- to-8-week-old STING N153S, WT littermate, Ifngr1 –/–, Rag1 –/–, and STING goldenticket (GT) mice that were inoculated intranasally with 2 × 10 5 PFU of γHV68. Survival was assessed for 60 days ...
WebThe loss of responsiveness in these mice ( Goldenticket ) was due to a destabilizing point mutation in STING. We isolated fibroblasts from wild-type and Goldenticket mouse … WebGoldenticket mouse embryonic fibroblast cells expressing hmSTING and mSTINGmutLoop constructs can support DENV2 replication. (A) mSTING restricts DENV2 replication in goldenticket MEFs. mRNA level of DENV2 NS5 was assessed by RT-qPCR after empty vector and mSTING construct were transfected into STING-KO MEFs after 24 h and then …
WebMar 6, 2024 · The N-ethyl-N-nitrosourea-induced Goldenticket mouse mutant reveals an essential function of Sting in the in vivo interferon response to Listeria monocytogenes and cyclic dinucleotides. Infect. … WebType I interferons (IFNs) are central regulators of the innate and adaptive immune responses to viral and bacterial infections. Type I IFNs are induced upon cytosolic …
WebSting gt (Goldenticket) mice have a missense mutation (I199N) rendering them deficient in Sting-dependent interferon responses to cyclic dinucleotides or Listeria infection (45). When treated with ...
WebJul 7, 2024 · STING S365A mice exhibit normal susceptibility to Mycobacterium tuberculosis infection but are unexpectedly much more resistant to HSV-1 as compared with STING … bird of the feather flock togetherWebApr 30, 2013 · MLFs derived from STING-deficient (Goldenticket) mice did not induce type I IFNs in response to C. trachomatis infection (Fig. 3B). Importantly, this defect in IFN expression by Goldenticket MLFs can be complemented with a wild-type copy of STING but not with a mutant form of STING (STING R231A ) that binds cyclic dinucleotides but … damned castWebApr 15, 2015 · Palpable B16 tumors were treated in wild-type (WT) and goldenticket (STING −/−) mice. The animals were treated with STINGVAX (20 μg of CDA), and the … bird of the feather songWeb025805 B6(Cg)- Sting1 tm1.2Camb /J MPYS -/- (STING KO) mice may be useful for studying cytosolic detection of pathogen DNA, the innate immune response and … damned creedWebMay 18, 2024 · largely curative outcomes, even after tumor re-challenge, in mouse models of melanoma and colon cancer and to survival benet in a preclinical murine breast cancer model. The NPs activated STING ... (goldenticket) mice. Finally, we provide fundamental insights into the intercommunication between cancer cells and immune cells in the context damned cracked multiplayerWebMay 23, 2013 · (C) HEK 293T cells were transfected with the indicated amounts of various mouse (m) or human (h) STING expression plasmid and then stimulated 6 hr later by transfection with synthetic cdG (5 μM). GT denotes the null I199N allele of STING from Goldenticket (Gt) mice. STING activation was assessed with the use of a cotransfected … damned call of duty zombiesWebDec 6, 2024 · wild-type (C57BL/6) mice were purchased from the National Laboratory Animal Center, Nakornpathom, Thailand. The Sting gt/gt mice (the goldenticket or Tmem173 gt) mice were created via chemically inducing mutagen with N-ethyl-N-nitrosourea (ENU) (22). Mice were bred and housed at the Faculty of Medicine, … bird of the day calendar